Genetic modification in micro-organisms
(b) Recombinant DNA technology, plasmids and artificial chromosomes.
Restriction endonucleases, marker genes, restriction sites, origin of replication, selective markers and regulatory sequences. Use of ligase in recombinant DNA. Genes can be introduced to confer an advantage. As a safety mechanism, genes are often introduced that prevent the survival of the microorganism in an external environment. Control of gene expression in recombinant plasmids and artificial chromosomes. Use of recombinant yeast cells to avoid polypeptides being folded incorrectly or lacking post translational modifications.
Revision Resources:
 |
 |
 |
|
|
Homework Sheets : CfE Higher Sht2_16
